- Seann William Scott Bo Duke
- Johnny Knoxville Luke Duke
- Jessica Simpson Daisy Duke
- Burt Reynolds Boss Hogg
- Willie Nelson Tío Jesse
- David Koechner Cooter Davenport
- Joe Don Baker Gobernador Jim Applewhite
- Lynda Carter Pauline
- Alice Greczyn Laurie Pullman
- Michael Weston Diputado Enos Strate