- Brad Pitt Teniente Aldo Raine
- Mélanie Laurent Shosanna Dreyfus
- Christoph Waltz Coronel Landa
- Eli Roth Donny Donowitz
- Michael Fassbender Archie Hicox
- Diane Kruger Bridget Von Hammersmark
- Daniel Brühl Frederick Zoller
- Til Schweiger Hugo Stiglitz
- Gedeon Burkhard Wilhelm Wicki
- Jacky Ido Marcel
- B.J. Novak Smithson Utivitch
- Omar Doom Omar Ulmer
- August Diehl Hellstrom
- Sylvester Groth Joseph Goebbels
- Martin Wuttke Adolph Hitler
- Mike Myers Ed Fenech
- Julie Dreyfus Francesca Mondino
- Samm Levine Soldado Hirschberg
- Paul Rust Andy Kagan
- Michael Bacall Soldado Michael Zimmerman
- Cloris Leachman Sra. Himmelstein
- Christian Berkel Eric
- Samuel L. Jackson Narrador
- Maggie Cheung Madame Mimieux
- Léa Seydoux Joven Shosanna
- Ken Duken Soldado alemán
- Alexander Fehling Master Sgt. Wilhelm / Pola Negri
- Denis Ménochet Perrier LaPadite