- Cate Blanchett Elizabeth I de Inglaterra
- Clive Owen Sir Walter Raleigh
- Geoffrey Rush Sir Francis Walsingham
- Jordi Mollà Felipe II
- Abbie Cornish Bess Throckmorton
- Samantha Morton María Estuardo
- Rhys Ifans Robert Reston
- Tom Hollander Sir Amyas Paulet
- Eddie Redmayne Babington
- Rosalind Halstead Mujer de la Corte
- Christian Brassington Archduke Charles