- Nia Vardalos Toula
- John Corbett Ian
- Michael Constantine Gus
- Lainie Kazan Maria
- Andrea Martin Tia Voula
- Joey Fatone Angelo
- Gia Carides Nikki
- Louis Mandylor Nick
- Ian Gomez Mike
- Jayne Eastwood Sra. White
- Bruce Gray Rodney Miller
- Bess Meisler Yiayia
- Fiona Reid Harriet Miller
- Rob Riggle Northwestern Rep
- Rita Wilson Anna
- Mark Margolis Panos
- John Stamos George