- Milla Jovovich Alice
- Iain Glen Dr. Isaacs
- Ali Larter Claire Redfield
- Eoin Macken Doc
- Ruby Rose Abigail
- Shawn Roberts Albert Wesker
- William Levy Christian
- Fraser James Razor
- Rola Cobalt
- Lee Joon-gi Comandante Lee
- Ever Anderson Alice (joven) / Red Queen