- Scarlett Johansson Natasha Romanoff / Viuda Negra
- Chris Evans Steve Rogers / Capitán América
- Robert Downey Jr. Tony Stark / Iron Man
- Elizabeth Olsen Wanda Maximoff / Bruja Escarlata
- Leslie Bibb Christine Everhart
- Paul Rudd Scott Lang / Ant-Man
- Marisa Tomei Aunt May
- Anthony Mackie Sam Wilson / Halcón
- Jeremy Renner Clint Barton / Ojo de Halcón
- Paul Bettany La Visión
- Sebastian Stan Bucky Barnes / Soldado de Invierno
- Tom Holland Peter Parker / Spider-Man
- Martin Freeman Everett Ross
- Emily VanCamp Sharon Carter / Agent 13
- Daniel Brühl Baron Zemo
- Don Cheadle Coronel James "Rhodey" Rhodes / Máquina de Guerra
- Chadwick Boseman T'Challa / Pantera Negra
- Frank Grillo Brock Rumlow / Crossbones
- William Hurt General Thaddeus 'Thunderbolt' Ross
- Gwyneth Paltrow Pepper Potts
- Stan Lee
- Jane Wu UN Staffer Wu