- Chris Pratt Peter Quill / Star-Lord
- Zoe Saldana Gamora
- Dave Bautista Drax
- Vin Diesel Baby Groot (voz)
- Bradley Cooper Rocket (voz)
- Michael Rooker Yondu
- Karen Gillan Nebula
- Pom Klementieff Mantis
- Elizabeth Debicki Ayesha
- Tommy Flanagan Tulik
- Sylvester Stallone Stakar
- Kurt Russell Ego
- Chris Sullivan Taserface
- Sean Gunn Kraglin
- Laura Haddock Meredith Quill
- Wyatt Oleff Peter Quill (joven)