- Chris Hemsworth Thor
- Karen Gillan Nebula
- Tom Holland Peter Parker / Spider-Man
- Scarlett Johansson Natasha Romanoff / Black Widow
- Chris Pratt Peter Quill / Star-Lord
- Tom Hiddleston Loki
- Elizabeth Olsen Wanda Maximoff / Scarlet Witch
- Dave Bautista Drax
- Pom Klementieff Mantis
- Zoe Saldana Gamora
- Robert Downey Jr. Tony Stark / Iron Man
- Benedict Cumberbatch Dr. Stephen Strange
- Chris Evans Steve Rogers
- Gwyneth Paltrow Pepper Potts
- Tessa Thompson Valkyrie
- Kerry Condon Friday
- Jon Favreau Happy Hogan
- Angela Bassett Ramonda
- Josh Brolin Thanos
- Vin Diesel Groot (voz)
- Jeremy Renner Clint Barton / Hawkeye
- Paul Rudd Scott Lang / Ant-Man
- Bradley Cooper Rocket Raccoon (voz)
- Benicio Del Toro The Collector
- Paul Bettany Vision
- Mark Ruffalo Bruce Banner / Hulk
- Benedict Wong Wong
- Terry Notary Cull Obsidian / Teen Groot
- Anthony Mackie Sam Wilson / Falcon
- Don Cheadle James Rhodes / War Machine
- Sean Gunn Rocket Raccoon
- Callan Mulvey Jack Rollins
- Jacob Batalon Ned
- Ariana Greenblatt Gamora (joven)
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