- Robert Downey Jr. Tony Stark / Iron Man
- Chris Evans Steve Rogers / Captain America
- Chris Hemsworth Thor
- Scarlett Johansson Natasha Romanoff / Viuda negra
- Mark Ruffalo Bruce Banner / Hulk
- Jeremy Renner Clint Barton / Ojo de Halcón
- Brie Larson Carol Danvers / Capitana Marvel
- Paul Rudd Scott Lang / Ant-Man
- Don Cheadle James Rhodes / Máquina de Guerra
- Danai Gurira Okoye
- Bradley Cooper Rocket (voz)
- Karen Gillan Nebula
- Gwyneth Paltrow Pepper Potts
- Josh Brolin Thanos
- Tessa Thompson Valkyrie
- Benedict Wong Wong
- Evangeline Lilly Hope van Dyne / The Wasp
- Jon Favreau Happy Hogan
- Michelle Pfeiffer Janet Van Dyne
- Elizabeth Olsen Wanda Maximoff / Scarlet Witch
- Chadwick Boseman T'Challa / Black Panther
- Sebastian Stan Bucky Barnes / Winter Soldier
- Pom Klementieff Mantis
- Tilda Swinton The Ancient One
- Dave Bautista Drax
- Letitia Wright Shuri
- Frank Grillo Brock Rumlow / Crossbones
- Ty Simpkins Harley Keener
- Hiroyuki Sanada Akihiko
- Terry Notary On-Set Groot / Cull Obsidian
- Angela Bassett Ramonda
- Michael Douglas Hank Pym
- Marisa Tomei Tía May
- Hayley Atwell Peggy Carter
- Natalie Portman Jane Foster
- John Slattery Howard Stark
- Tom Hiddleston Loki
- Anthony Mackie Sam Wilson / Falcon
- Rene Russo Frigga
- Zoe Saldana Gamora
- Tom Holland Peter Parker / Spider-Man
- Benedict Cumberbatch Stephen Strange / Doctor Strange
- William Hurt Secretario de estado Thaddeus Ross
- Cobie Smulders Maria Hill
- Sean Gunn On-Set Rocket / Kraglin
- Linda Cardellini Laura Barton
- James D'Arcy Jarvis
- Vin Diesel Groot (voz)
- Robert Redford Alexander Pierce
- Chris Pratt Peter Quill / Star-Lord
- Samuel L. Jackson Nick Fury
- Kerry Condon Friday (voz)
- Stan Lee Conductor
- Callan Mulvey Jack Rollins
- Ken Jeong Guarda de seguridad
- Jackson A. Dunn Scott Lang (12 años)
- Jacob Batalon Ned
- Taika Waititi Korg (voz)
- Jack Champion Chico en bici